At the AACR Annual Meeting 2023, PreOmics GmbH unveiled the revolutionary BeatBox-based FFPE workflow, revolutionizing the world of tissue proteomics. This ground-breaking new workflow simplifies, speeds up and standardizes FFPE sample preparation, eliminating the need for xylene-based deparaffinization and enabling fast and robust processing of up to 96 samples in parallel for discovery research, as well as for larger cohort translational and preclinical research and validation. With BeatBox, deep and unbiased tissue proteomics are now achievable like never before.
The BeatBox™ tissue homogenizer and cell lyser is a revolutionary, benchtop system that makes semi-automated sample homogenization fast and easy. Offering flexible input amounts ranging from 1-50 mg, this device guarantees reproducible results for LC-MS-based proteomics with scalable throughput from 1-96 samples.
Ideal for fresh tissue and cell-line proteomics, the BeatBox now also offers a novel FFPE protocol for efficient processing of FFPE samples for downstream tissue proteomics. With no complex handling or water conditioning required and a homogenization time of only 10 minutes, the BeatBox is the perfect solution for convenient and reliable sample homogenization.
The novel BeatBox FFPE workflow was recently put to the test with a benchmark study, where it was compared to a sonication approach on FFPE as well as fresh frozen mouse cardiac muscle, kidney, and liver tissue.
Using a timsTOF-HT mass spectrometer in DIA-PASEF mode, the BeatBox workflow significantly increased the proteomic depth of both FFPE and fresh frozen tissue, ranging from an average of 14% to 43%.
In addition to its impressive results, the BeatBox approach was also shown to be highly repeatable, with typical Coefficients of Variation (CV) of less than 10%. On top of that, this novel approach is a time-saver, taking up to four hours less than the sonication workflow, allowing for parallelized, high-throughput processing of up to 96 samples in one working day.
Dr. Michael Wierer and his team at the University of Copenhagen have made a landmark discovery that could revolutionize pathology research. By successfully adapting the BeatBox technology for tissue homogenization, they have managed to efficiently homogenize paraffin-embedded tissue scrolls.
This breakthrough allows them to process large cohorts of FFPE tissue quickly, reproducibly, and with greater precision, making large-scale biobank projects a reality. Thanks to this innovation, researchers can now uncover novel deep proteomics insights that have the potential to transform the field of pathology research.
Dr. Marcello Stein, Chief Marketing Officer of PreOmics, has declared a revolutionary breakthrough in FFPE sample preparation with the introduction of the BeatBox-based workflow. This new method eliminates the need for xylene-based deparaffinization, allowing researchers to simplify, speed-up and standardize FFPE sample preparation. This major advancement in proteomic analysis opens the door to unprecedented levels of unbiased and deep proteomics across large sample cohorts.
Bruker Corporation (Nasdaq: BRKR) and PreOmics have joined forces to bring a revolutionary solution to the market: tissue workflows powered by BeatBox, now available for purchase with Bruker’s timsTOF Pro mass spectrometer. This cutting-edge technology allows for greater accuracy and efficiency in mass spectrometry, and is sure to revolutionize the field.
About PreOmics GmbH
At PreOmics, we believe that life sciences should have access to reliable, efficient solutions and workflows to uncover biological knowledge. Our energy, commitment, and team spirit make us a highly creative and quality-focused partner with extensive scientific experience.
We strive to make revolutionary proteomic discovery processes available to everyone, so that we can uncover the hidden causes of diseases, ensure sustainable nutrition, and provide accurate diagnoses that will improve lives and society.